ANIMAL USE POLICY
Animals are used for experiments in the Biosci labs. Please see the lab
director or course instructor if you have objections to working with
animals in the labs.
Teaching Assistants:
TUESDAY LAB: Juliesta Sylvester
<juliestasylvester@mac.com>
WEDNESDAY LAB: Daniel Rabe <drabe@uchicago.edu>
THURSDAY LAB: Steven Droho <sdroho@uchicago.edu>
FRIDAY LAB: Amit Sharma <sharma@uchicago.edu>
| Lab handouts will download as PDF files. To view pdf files you need to have Adobe Acrobat® Reader installed on your computer. To install the free reader, click on the "Get Acrobat® Reader" button. | |
WEEK 1 - LAB BASICS
LabBasics
Handout - please bring a copy of the handout to lab.
For this week only, the lab is divided into two sessions.
Students with last names A-L show up at 1:30 while students with last
names M-Z show up at 3:30.
Weeks 2-8, labs begin at 1:30 for all students.
Pipetting Basics MovieWEEK 2 - FLUORESCENCE MICROSCOPY
Microscopy Lab HandoutWEEK 3 - DNA Mapping Lab
Microscopy PreLab Quiz
DNA Mapping Lab Handout
DNA Mapping PreLab Quiz (You have to prepare a graph for this quiz so do not wait until just before lab)
Gel Electrophoresis Movie (Please watch the movie before lab)Generic gels (you can use these for your lab report only if your own gels did not work)
Polytene Chromosome Lab HandoutWEEK 5- NO LAB
Polytene Chromosome PreLab Quiz
Polytene Chromosome Dissection Movie (please watch the movie before lab)EXTRA Credit Papers (see lab handout for details) on the "histone code"
Jenuwein and Allis Review
Dutnall Review
Turner Review (has nice illustrations)
FOR ALL GRAPHS, annotate the graphs, indicating with arrows where
the
different reagents (mitos, succinate, ADP, etc.) were added. See
the examples in the lab handout.
Also, please remember to answer the questions at the ends of the
protocols
(at least for protocol #1 and 2).
WEEK 8 - Cytoskeleton Lab
Salivary Gland Polytene
chromosomes